transwell apparatus model no: 3450  (Corning Life Sciences)

Journal: The Journal of Biological Chemistry

Article Title: ANP32A-mediated histone 3 K27 acetylation is essential for sotorasib activity in KRAS-mutant non–small cell lung cancer

doi: 10.1016/j.jbc.2025.111110

Figure Lengend Snippet: ANP32A promotes the proliferation of KRAS -mutant lung cancer cells. A , Western blot analysis on ANP32A protein in control (sgCtrl) and knockout (sgANP) H2030 and H358 cells. B and C , CCK-8 assay measured cell proliferation in sgCtrl and sgANP H2030 ( B , ∗p = 0.024, ∗∗p = 0.007) and H358 ( C , ∗∗p = 0.002 versus day 2, 0.006 versus day 3) cells as indicated (n = 3). D – F , statistical analyses of EdU ( D , ∗p = 0.03, ∗∗∗p < 0.001), Transwell ( E , ∗∗∗p < 0.001), and cell cycle profile ( F ) in sgCtrl and sg ANP H2030 and H358 cells as indicated (n = 3). Percentage of G1/G0 ( top panel , ∗∗p = 0.009, ∗∗∗p < 0.001) and G2/M ( bottom panel , ∗p = 0.024, ∗∗p = 0.001) was shown in F . G , Western blot analysis on ANP32A protein in control (shCtrl), knockdown (shANP), and knockdown with ANP32A reintroduction (shANP + ANP) H2030 and H358 cells. H and I , CCK-8 assay measured cell proliferation in shCtrl, shANP, shANP + ANP H2030 ( H , ∗∗∗p < 0.001, ###p < 0.001) and H358 ( I , ∗∗p = 0.002 versus day 2, 0.002 versus day 3; #p = 0.018 versus day 2, 0.034 versus day 3) cells as indicated (n = 3). J – L , statistical analyses of EdU ( J , ∗p = 0.013 versus shCtrl, 0.031 versus shANP; ∗∗ p = 0.008 versus shCtrl, 0.007 versus shANP), Transwell ( K , ∗∗∗p < 0.001), and cell cycle profile ( L ) in shCtrl, shANP, shANP + ANP H2030 and H358 cells as indicated (n = 3). Percentage of G1/G0 ( top panel , ∗∗∗p < 0.001) and G2/M ( bottom panel , ∗p = 0.029 versus shCtrl, 0.01 versus shANP, ∗∗ p = 0.002, ∗∗∗ p < 0.001) is shown in L . ∗ indicates p < 0.05, ∗∗ indicates p < 0.01, and ∗∗∗ indicates p < 0.001; # indicates p < 0.05, ## indicates p < 0.01, and ### indicates p < 0.001 compared with the shANP group in ( H ) and ( I ). CCK-8, Cell Counting Kit-8; Edu, 5-ethynyl-2′-deoxyuridine.

Article Snippet: A total of 2 × 10 4 cells were suspended in a serum-free medium and placed in the upper chamber of a Transwell apparatus (84052ES12; Yeasen Biotechnology).

Techniques: Mutagenesis, Western Blot, Control, Knock-Out, CCK-8 Assay, Knockdown, Cell Counting

Journal: Drug Design, Development and Therapy

Article Title: Jingfang Granules for Diabetic Wound Healing: Insights from Network Pharmacology and Experimental Validation

doi: 10.2147/DDDT.S516298

Figure Lengend Snippet: JFG serum promotes angiogenesis in HUVECs. ( A ) Wound healing assay of HUVECs with blank serum or JFG serum incubated for 24 and 48 h (scale bars, 500 μm). ( B and C ) Quantification of the migration rate of 24 and 48 h measured by ImageJ. ( D ) Effect of JFG serum on the vertical migratory capacity of HUVECs in the transwell assay (scale bars, 100 μm). ( E ) Cells migrating to the lower chambers were stained with crystal violet and the absorbance was determined. ( F ) Effect of JFG serum on tube formation of HUVECs (scale bars, 500 μm). ( G ) Number of nodes connecting individual complete capillaries. Data are expressed as mean ± SD of independent experiments, n=6. * p < 0.05, ** p < 0.01 vs blank serum group; “ns” indicates “not significant” ( p > 0.05).

Article Snippet: The effect of JFG serum on the longitudinal migration of HUVECs was determined using an 8 μm pore polycarbonate membrane transwell apparatus (Corning, New York, USA) as previously described.

Techniques: Wound Healing Assay, Incubation, Migration, Transwell Assay, Staining